Fipronil ELISA Test Kit

Product Description

REAGEN^™ Fipronil ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fipronil in fish, shrimp ,meat , milk ,eggs and honey.

The unique features of the kit are:

1. high recovery (80%-105%), and cost-effective extraction methods for various samples.

2. Low detection limit (0.05 ng/g or ppb) for various samples.

3. High reproducibility.

4. A quick ELISA assay (less than 1hours regardless of number of samples).

Procedure Overview

REAGEN^™The method is based on a competitive colorimetric ELISA assay. The Fipronil antibody has been coated in the plate wells. During the analysis, sample and Fipronil-horseradish peroxidase ( Fipronil-HRP) conjugate are added to the wells for incubation. If the Fipronil residue is present in the sample, it will compete for the Fipronil antibody, thereby preventing the Fipronil-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the Fipronil residue concentration in the sample.

assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Sensitivity (Detection Limit)

Specificity (Cross-Reactivity)